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实验设计

Bioscreen C每次可测200个样。在高温条件下(高温发酵)测量生长曲线只需23 h。细菌(如E.coli)在37 ℃条件下测量生长曲线只需418 h

 

微生物在Bioscreen C中生长时比在培养皿中要快2倍。如果进行振荡培养,甚至要比固体培养基快4倍。微生物在冰箱中生长很慢,测量其生长曲线需要长达1个星期之久。

     可以在晚上离开实验室前将Bioscreen C实验准备好并开始运行,第二天早晨回到实验室时200个生长曲线已经做好。

 

可在Bioscreen C上使用的常见培养基

Arginine Broth, Azide Dextrose Broth, Bolton Selective Enrichment Broth, Brain Heart Broth, Brilliant-green 2 %-Bile Broth, Bromocresol-purple Azide Broth, Buffered Listeria Enrichment Broth, Casein-peptone Lecithin Polysorbate Broth, CASO Broth (Casein-peptone Soymeal-peptone Broth), DEV Dextrose Broth, DEV Glutamate Broth, DEV Lactose Broth, DEV Lactose Peptone Broth, DEV Tryptophan Broth, Differential Reinforced Clostridial Broth (DRCM), EC Broth, Enterobacteriaceae Enrichment Broth, FRASER Listeria Selective Enrichment Broth, Staphylococcus Enrichment Broth, GN Enrichment Broth, ITC (Irgasan Ticarcillin Chlorate) Broth, Lactose Broth, Lauryl Sulfate Broth, LB-Broth (LENNOX), LB-Broth (MILLER), Letheen Broth, Listeria Enrichment Broth (LEB), M-(Mannose) Broth, MacCONKEY Broth, Malachite-green Broth, Malt Extract Broth, mEC Broth with Novobiocin, MRS Broth (Lactobacillus Broth), MR-VP Broth (Methyl-red VOGES-PROSKAUER Broth), mTSB Broth with Novobiocin, MUELLER-HINTON Broth, Nutrient Broth, L-PALCAM Listeria Selective Enrichment Broth, Phenol-red Broth Base , SABOURAUD-2 % Dextrose Broth , Salmonella Enrichment Broth, Salmonella Enrichment Broth, Salmosyst Broth Base , Selenite Cystine Broth , Selenite Enrichment Broth, Sodium chloride peptone broth (buffered) , Standard I Nutrient Broth , Staphylococcus Enrichment Broth Base, TBG-Broth (Tetrathionate-Brilliant-green Bile Enrichment Broth), Tetrathionate Broth, Tetrathionate Broth Base, Tetrathionate Crystal-violet Enrichment Broth, Thioglycollate Broth , Tryptic Soy (CASO) Broth, Tryptic Soy Broth (TSB), Tryptose Broth , Tyrobutyricum Broth Base , Urea Broth , UVM-Listeria Selective Enrichment Broth, Vitamin B12 (Lactobacillus) Assay Broth, Vitamin Biotin Assay Broth , Vitamin Folic Acid Assay Broth, Vitamin Nicotinic Acid Assay Broth , Vitamin Pantothenic Acid Assay Broth, Wort Broth, Yersinia Selective Enrichment Broth

 

全生长曲线分析仪新的发现及用户意见

1、发现新的变种

美国Cornell University 发现了熟悉的微生物的新变种。检查这种微生物的生长曲线发现在一个下降后突然有一个快速的增长。进一步的检测发现这是个新变种。这种不同寻常的生长曲线形状是观察这种变化的有效手段。

2、用彩色纸来快速区分各个微孔

在纸上将所画的各个孔涂上不同的颜色能够将微孔板的各个孔快区分开来这样在用移液器加样时就不会弄错。

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